产品简介 Species Human Protein Construction Expressed with an N-terminal Met. IL-6 (Pro29-Met212)Accession # Q75MH2 Purity > 95% as analyzed by SDS-PAGE Endotoxin Level < 0.2 EU/μg of protein by gel clotting method Biological Activity ED50 < 1.0 ng/ml, measured in a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 1.0 × 106 U/mg. Expression System E. coli Apparent Molecular Weight ~20.9 kDa, on SDS-PAGE under reducing conditions. Formulation Lyophilized after extensive dialysis against PBS. Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. Storage & Stability Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.