A01724 THE™ V5 Tag Antibody, mAb, Mouse

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A01724 THE™ V5 Tag Antibody, mAb, Mouse

产品概述
SpecificityTHE™ V5 Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.
Host SpeciesMouse
ImmunogenV5 tag peptide conjugated to KLH
ConjugateUnconjugated

应用指导
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ApplicationRecommended Usage
ELISA0.05-0.1 µg/ml
Western Blot0.2-1 µg/ml
Immunoprecipitation (IP)1-5 µg/ml
Flow Cytometry1-3 µg for 1 x 106 cells
Immunocytochemistry/Immunofluorescence (ICC/IF)1-3 µg/ml
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产品属性
FormLyophilized
Storage Bufferlyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
ReconstitutionReconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage InstructionsThe lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles.
PurificationProtein A affinity column
IsotypeMouse IgG2a,κ
ClonalityMonoclonal
Clone ID4C12E11