L00766 MonoRab™ Anti-DYKDDDDK Affinity Resin
L00766 MonoRab™ Anti-DYKDDDDK Affinity Resin
产品概述
| Product Description | DYKDDDDK or FLAG® tag is an octapeptide that can be added to a protein using recombinant DNA technology. The tag sequence can be fused to the coding sequence of a target protein to facilitate detection and purification. GenScript’s MonoRab™ Anti-DYKDDDDK Affinity Resin (Cat. No. L00766) is designed for optimal purification of DYKDDDDK-tagged proteins. Based on our new rabbit monoclonal antibody technology (MonoRab™), this resin offers high binding capacity, high purity, and resistance to high concentrations of salt or acid. The binding capacity of MonoRab™ Anti-DYKDDDDK Affinity Resin is 1.5 mg DYKDDDDK-tagged protein per mL of settled resin. The recombinant proteins expressed in bacterial or mammalian cells cultures specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin, resulting in highly pure eluates. The resin can withstand stringent washing steps, especially with buffers containing high concentration of salt. The resin is resistant to up to 2 M NaCl, and to buffers with pH as low as 2.5. Table 1 lists the main characteristics of the MonoRab™ Anti-DYKDDDDK Affinity Resin. |
产品属性
| Product Content | 50% settled resin in TBS with 50% glycerol and 0.02% sodium azide |
| Matrix | 4% cross-linked agarose |
| Average Bead size | 90 μm |
| Ligand | MonoRab™ monoclonal antibody against DYKDDDDK tag |
| Binding Capacity | Approximately 1.5 mg DYKDDDDK-tagged protein (Size: 50KDa) per ml settled resin |
| Elution Method | Two methods can be implied for elution:1. Alkaline, Elution under native conditions by competition with peptide2. Elution with SDS-PAGE loading buffer*. |
| Resin Reuse | The resin can be recycled for at least 4 times. If maintained as per manufacturer’s instructions, the resin can be reused 10 times with minimum loss of binding capability |
| Reagents Compatibility | Compatible with commonly used reagents at certain concentrations |
| Storage & Stability | Store at -20 °C for 12 months. |
