L00863 CytoSinct™ CD4 Nanobeads, human
L00863 CytoSinct™ CD4 Nanobeads, human
| Product Description | CytoSinct CD4 Nanobeads, human is used for in vitro enrichment of CD4+ cells from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products, or single-cell suspension based on the surface expression of human CD4. To begin the isolation, Nanobeads are added to the cells. The cells/beads mixed suspension is loaded onto a CytoSinct column placed in the CytoSinct magnet, or other compatible columns with a magnet. The CD4+ cells labeled with Nanobeads are retained within the column and unlabelled cells (CD4- cells) are flushed out during the wash step. After removing the column from the magnetic field, the retained CD4+ cells can be eluted as the positively selected cell fraction. |
| Format | Supplied in phosphate-buffered saline (PBS), containing Human Serum Albumin (HSA) and Poloxamer 188. | |||||||||
| Components | 1 mL or 0.5 mL CytoSinct CD4 Nanobeads, human Nanobeads conjugated to monoclonal anti-human CD4 antibodies (isotype: mouse IgG1). | |||||||||
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| Storage | Store at 2 - 8 °C. Do not freeze. |
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CD4+ T cells were enriched from human PBMCs. CD4+ cells were stained with anti-CD4 (clone: SK3) APC and gated on Live/hCD45+.»
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
