Interleukin 1 beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.

Species：Human

Protein Construction：IL-1β (Ala117-Ser269)

                                     Accession # P01584

Purity：> 95% as analyzed by SDS-PAGE

             > 95% as analyzed by HPLC

Endotoxin Level：< 0.2 EU/μg of protein by gel clotting method

Biological Activity：ED50 < 10.0 pg/ml, measured in a cell proliferation assay using D10S cells, corresponding to a specific activity of >1.0 × 108 units/mg.

Expression System：CHO

Apparent Molecular Weight：~17 kDa, on SDS-PAGE under non-reducing conditions.

Formulation：Lyophilized after extensive dialysis against PBS.

Reconstitution：It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.

Storage & Stability：Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

技术背景

Target Background：Interleukin 1 beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.

Synonyms：IL1B; IL-1; IL1-BETA; IL1F2; interleukin 1 beta; IL1beta

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.