| Species | Mouse |
| Protein Construction | Expressed with an N-terminal Met. M-CSF (Lys33-Pro187)
Accession # P07141 |
|
| Purity | > 95% as analyzed by SDS-PAGE |
| Endotoxin Level | < 0.2 EU/μg of protein by gel clotting method |
| Biological Activity | ED50 < 3.0 ng/ml, measured in a cell proliferation assay using Murine M-NFS-60 cells, corresponding to a specific activity of > 3.3 × 105 U/mg. |
| Expression System | E. coli |
| Apparent Molecular Weight | ~30 kDa, on SDS-PAGE under non-reducing conditions. |
| Formulation | Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0. |
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
| Storage & Stability | Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
