Enceed™ T cell Activation, human
Enceed™ T cell Activation, human
| Product Description | The Enceed T cell Activation reagent induces T cell activation and expansion in enriched T cells or PBMCs. The Enceed T cell Activation reagent is composed of biodegradable matrix-coated nanoparticles conjugated with mouse anti-human CD3 and mouse anti-human CD28 antibodies. The small sized particulate structure of the activation reagent enables its easy removal by media washing or centrifugation. The recommended titer of 1:200 has been found to achieve efficient stimulation of majority of T cell subsets. For special applications, the optimal stimulation titer should be determined experimentally. Excessive amount of the Enceed T cell Activation reagent may result in over-activation of T cells risking activation-induced cell death. Typically, the cells reach the exponential growth phase over 5 days and keep optimal expansion up to 13 days. Restimulation after 13 days can be performed for longer cultivation. The Enceed T cell Activation reagent can be used together with human IL-2, other cytokines such as human IL-15 and IL-7 can also be added for more efficient activation. |
| Format | Supplied in phosphate-buffered saline (PBS), containing Human Serum Albumin (HSA) and Poloxamer 188. | |||||||||
| Components | 1 mL or 0.5 mL Enceed T cell Activation, human Nanobeads conjugated to monoclonal anti-human CD3 and anti-human CD28 antibodies | |||||||||
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| Storage & Stability | Store at 2-8 °C. Do not freeze. |
