GenCRISPR™ LbuCas13a Nuclease Z03742
GenCRISPR™ LbuCas13a Nuclease Z03742
| Description | GenCRISPR™ LbuCas13a Nuclease is an RNA-guided RNA endonuclease from Leptotrichia buccalis. Cas13a (previously known as C2c2) belongs to the Class 2 Type VI CRISPR-Cas system and contains a single protein effector with two HEPN domains. Cas13a exhibits two distinct ribonuclease activities. Cas13a possesses sequence-specific RNA cleavage activity in vitro and in vivo. And after specific cleavage, Cas13a activates collateral cleavage activity towards adjacent non-specific ssRNA sequences. The two distinct ribonuclease activities of Cas13a enable guide-RNA processing and RNA detection. The RNA detection empowered with Cas13a-crRNA complex and quenched fluorescent ssRNA as reporter has been reported to be versatile, rapid and high-specific. |
| crRNA-dependent RNA cleavage |
| Source | Recombinant Cas13a expressed by E.coli |
| Species | Leptotrichia buccalis |
| Tag | Tag-free |
| Apparent Molecular Weight | ~140 kDa, on SDS-PAGE under non-reducing conditions |
| Concentration | 4 mg/ml |
| Storage Buffer | 50 mM Tris-HCI, 600 mM NaCl, 0.1 mM TCEP, 50% glycerol, pH 7.5. |
| Storage & Stability | Store at -20 °C for 12 months from the date of manufacture. Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
| Accession# | C7NBY4 |
| Appearance | Clear, colorless liquid |
| Purity | ≥ 90% by SDS-PAGE |
| Activity | ≥ 85% by in vitro digestion of ssRNA |
| Concentration | 4 mg/ml ± 10% as analyzed by A280 |
| Residual DNase | Undetectable |
| Residual RNase | Undetectable |
| Endotoxin Level | < 0.2 EU/μg |
