Cas9 ELISA Kit L01069

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Cas9 ELISA Kit L01069

Linearity Range0.125 to 8 ng/mL
SensitivityLLOQ:0.125ng/ml
PrecisionIntra-assay: CV ≤ 10%;
Inter-assay: CV ≤ 15%
Accuracy80%-120%
Standard3.2 µg/mL of Cas9 protein
Suitable Sample Typecell lysates, viral vectors, and gene-editing reagents
SpecificityThis kit is designed for the detection of a wide range of Cas9 proteins, including wild-type SpCas9, high-fidelity mutants (e.g., eSpCas9), and variants featuring nuclear localization signals (NLS) at the N-terminus, C-terminus, or both.
Assay Temperature37°C
Assay Time3 h
StorageThe unopened kit is stable for 12 months from the date of manufacture at 2°C to 8°C, and the opened kit is stable for 1 month from the date of opening at 2°C to 8°C.
Kit Contents
ComponentQuantity/SizePart No.
Cas9 Capture Plate1 plateB2-80
Cas9 Standard Stock1 vial (50 μL)B2-10
Biotin Anti-Cas9 Antibody1 bottle (12 mL)B2-20
Streptavidin-HRP1 bottle (12 mL)B2-30
Sample Dilution Buffer1 bottle (60 mL)B2-60
20× Wash Solution1 bottle (60 mL)B-A1-71
TMB Solution1 bottle (12 mL)B-A1-40
Stop Solution1 bottle (6 mL)B-A1-50
Plate Sealer2 pieces/


Cas9 ELISA Kit

Figure 1. Typical Cas9 standard curve fitted with a four-parameter logistic model.»

Cas9 ELISA Kit

Figure 2: Cas9 variants curves»

Cas9 ELISA Kit

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Cas9 ELISA Kit

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Cas9 ELISA Kit

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Cas9 ELISA Kit

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Cas9 ELISA Kit

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.