Anti-His Affinity Resin L00439
Anti-His Affinity Resin L00439
| Product description | GenScript Anti‐His Affinity Resin is designed for purification of His‐tagged protein that is difficult for single Ni‐NTA Agarose purification. This product is made from THE™ anti‐His monoclonal antibody conjugated to agarose resin. It specifically binds to His‐tagged proteins in the samples. Proteins nonspecifically binding to the resin can be eliminated by washing. Finally, His‐tagged protein can be eluted by different methods. In addition to that, the resin can be used for immunoprecipitation of His‐tagged proteins. |
| Product Content | 50% resin in TBS with 0.02% sodium azide |
| Beads Structure | 4% agarose |
| Beads Size | 45-165 μm, averagely 90 μm |
| Coupled Antibody | THE™ His Tag Antibody, mAb, Mouse (Cat.No A00186) |
| Binding Capacity | ≥0.6 mg His-tagged protein/ml Resin |
| Specificity | N‐terminal/C‐terminal/internal /4 x His/5 x His/6 x His‐tagged proteins |
| Elution Method | Acid, alkaline and peptide competitive elutions |
| Repeat Usage | Generally the resin can be recycled at least 4 times. If maintained properly, the resin may reach 10 recycle times with minor binding capability loss |
| Reagents Compatibility | Compatible with mostly used reagents at certain concentrations |
| Consistency | Lot‐to‐lot consistency proved by strict production procedure |
| Storage | This product is stable for 1 year if stored at 2°C~8°C from the date of manufacture. Do not store at 0°C or below 0°C to avoid freezing the resin. |
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
