| Product Description | Glutathione S-transferase (GST) Tag is engineered to create the GST gene fusion system, a system used to purify and detect proteins of interest. The GST Tag is composed of 220 amino acids, with a molecular weight of 26 kD and GST tagged proteins is expressed in high levels in bacteria expression system and the fusion protein is purified in a single step by affinity chromatography using glutathione agarose. With site-specific proteases, such as thrombin or factor Xa, the GST portion can easily be removed from GST tagged protein. GST tag antibody is a universal tool for detection of GST tagged protein.GenScript GST Tag ELISA Kit was developed for three purposes, to identify GST tagged protein, monitoring the protein expression and for screening the expression optimization of the protein. It can also detect residual GST in the GST tag cleavage during protein purification. However it is possible that improper folding of GST or the presence of a fusion partner may prevent GST binding with the GST monoclonal antibodies in this kit.The kit detects GST tagged protein in a sandwich immunoassay. It utilizes two clones of GST monoclonal antibodies that bind to different epitopes of the GST tag protein at the same time. The GST monoclonal antibody, pre-coated on the plate, is used to capture GST tagged protein and the biotin conjugated GST monoclonal antibody (Biotin-GST Mab) is added to interact with GST tagged protein bound on the plate. Streptavidin-HRP is added to interact with Biotin-GST Mab and the final step involves the addition of TMB Solution that will react with HRP to give product with an absorbance at 450 nm. The quantity of GST tagged protein in sample is determined by comparisons with the absorbance of that of a known GST tagged protein standard curve. |
| Key Features | High Sensitivity: 1 ng/ml GST tagged proteinBroad Detection Range: 3.125~100 ng/mlApplication: Identifying GST tagged protein Checking GST tagged protein expression and screening the expression optimization Detecting residual GST in the GST tag cleavage during protein purification Rapid quantificationFeasible Operation: Provide all reagents required for test Complete the test in an hour |
| Precision | Intra-assay Precision: CV<5%Inter-assay Precision: CV<10% |
| Storage | The unopened kit is stable for 12 months if stored at 2-8°C and the opened kit may be stable for 1 month at 2-8°C. Do not freeze the kit |
| Kit Contents | | Components | Quantity | Part.No |
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| GST Capture Plate | 1 plate (8 wells x 12 strips) | 411-80 | | Sample Dilution Buffer | 30 ml | 411-60 | | Biotin-GST Mab Stock | 200 µl | 411-20 | | Antibody Dilution Buffer | 15 ml | 411-90 | | Streptavidin-HRP | 12 ml | 411-30 | | GST Protein Stock (1.0 mg/ml) | 10 µl | 411-10 | | 20× Wash Solution | 40 ml | B-A1-70 | | TMB Solution | 12 ml | B-A1-40 | | Stop Solution | 6 ml | B-A1-50 | | Adhesive Plate Cover | 2 pieces | N/A |
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