| This product is suitable for immunoblotting (Western or Dot blot), ELISA, immunoperoxidase electron microscopy, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. The investigator must determine the ideal working concentration for each specific application. We have not yet determined the suitability of this antibody for applications other than those listed below. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application | Recommended Usage |
|---|
| Dot blot | 1:5,000-1:20,000 | | Western blot | 1:5,000-1:20,000 | | ELISA | 1:50,000-1:250,000 | | Immunohistology | 1:500-1:2,500 |
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