A00170 DYKDDDDK-tag Antibody, pAb, Rabbit

产品中心 > 免疫学 > A00170 DYKDDDDK-tag Antibody, pAb, Rabbit

A00170 DYKDDDDK-tag Antibody, pAb, Rabbit

产品概述
SpecificityThis Antibody recognizes DYKDDDDK tagged fusion proteins.
Host SpeciesRabbit
ImmunogenA synthetic peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH
ConjugateUnconjugated

应用指导
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
ApplicationRecommended Usage
ELISA0.01-0.05 µg/ml
Western Blot0.1-1.0 µg/ml
Western Blot1:100-1:1,000
Immunocytochemistry/Immunofluorescence (ICC/IF)5-20 µg/ml
Flow Cytometry1-3 µg for 1 x 106 cells
返回DYKDDDDK-tag Antibody, pAb, Rabbit

产品属性
FormLyophilized
Storage BufferLyophilized with PBS, pH 7.4, containing 0.02% sodium azide
ReconstitutionReconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage InstructionsThe lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles.
PurificationAffinity chromatography
IsotypeRabbit IgG
ClonalityPolyclonal
Clone IDNot applicable
技术背景
Target BackgroundThe DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.It has been used extensively as a general epitope tag in expression vectors.DYKDDDDK-tag Antibody, pAb, Rabbit can be used to detect DYKDDDDK-tagged proteins.
SynonymsRabbit Anti-DYKDDDDK-tag pAb;Rabbit Anti-Flag-tag pAb;


For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.