Z03025 IL-18, Rat

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Z03025 IL-18, Rat

产品简介
SpeciesRat
Protein Construction

Expressed with an N-terminal Met.

IL-18 (His37-Ser194)Accession # P97636-1
Purity> 95% as analyzed by SDS-PAGE
Endotoxin Level< 0.2 EU/μg of protein by gel clotting method
Biological ActivityED50 < 2.0 μg/ml resulted in the secretion of 0.15 ng/ml h-IFN-gamma when tested using KG-1 cell line, corresponding to a specific activity of > 500.0 U/mg.
Expression SystemE. coli
Apparent Molecular Weight~18.4 kDa, on SDS-PAGE under reducing conditions.
FormulationLyophilized after extensive dialysis against 50 mM Tris, 50 mM NaCl, pH 8.0.
ReconstitutionIt is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage & StabilityUpon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.