| Species | Human |
| Protein Construction | | IL-12 p35 (Arg23-Ser219)Accession # P29459 | | IL-12 p40 (Ile23-Ser328)Accession # P29460 |
|
| Purity | > 95% as analyzed by SDS-PAGE> 95% as analyzed by HPLC |
| Endotoxin Level | < 0.2 EU/μg of protein by gel clotting method |
| Biological Activity | ED50 < 2.0 ng/ml, measured by the induction of IFN-γ from NK cells, corresponding to a specific activity of > 5.0 × 105 U/mg. |
| Expression System | HEK 293 |
| Theoretical Molecular Weight | 75 kDa |
| Formulation | Lyophilized after extensive dialysis against PBS. |
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
| Storage & Stability | Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
