| Species | Mouse |
| Protein Construction | TNF-α (Leu80-Leu235)
Accession # P06804 |
|
| Purity | > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC |
| Endotoxin Level | < 0.2 EU/μg of protein by gel clotting method |
| Biological Activity | ED50 < 0.05 ng/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells, corresponding to a specific activity of > 2.0 × 107 U/mg. |
| Expression System | E. coli |
| Apparent Molecular Weight | ~17.3 kDa, on SDS-PAGE under reducing conditions. |
| Formulation | Lyophilized after extensive dialysis against PBS. |
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
| Storage & Stability | Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
