M00091 Streptavidin-HRP
M00091 Streptavidin-HRP
| Working concentrations for specific applications should be determined by titration assay.Appropriate concentrations will be affected by several factors including primary antibody, antigen amount, sensitivity of detection method, temperature, and incubation time, etc. The following dilution ranges are just a guideline: | |||||||||||
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| Formulation | Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal |
| Reconstitution | Reconstitute the lyophilized power with 1.0 ml of deionized water (or equivalent) to a final concentration of 1 mg/ml. |
| Storage | The product remains stable in lyophilized form for 1 year if stored at 2-8°C, -20°C, or lower from the date of receipt. For longer storage, -20°C or below is recommended. The reconstituted product can be stored for 2-3 weeks at 2-8°C or for 1 year at -20°C or below. Avoid repeated freezing and thawing cycles. If slight precipitation occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Target Background | Streptavidin is a protein that has similar binding properties to avidin. It is isolated from Streptomyces Avidinii. Streptavidin has a molecular weight of 60 KD and has 4 subunits. Each subunit can bind one molecule of biotin. Biotin is a 244 dalton water-soluble vitamin. Streptavidin has an extremely high binding affinity (Kd=10-15) for biotin. Streptavidin-HRP (M00091) is coupled streptavidin with horseradish peroxidase (HRP). This highly sensitive detector conjugate binds to biotinylated primary or secondary antibodies in enzyme immunoassay, immunoblotting, immunohistochemistry, flow cytometry, hybridoma screening systems. This conjugate also binds to nucleic acids labeled with biotinylated nucleotide analogs in Southern or Northern blot. |
For research use only. Not intended for human and animal therapeutic or diagnostic use.
