| Species | Human |
| Protein Construction | Expressed with an N-terminal Met. | EGF (Asn971-Arg1023)Accession # P01133 |
|
| Purity | > 95% as analyzed by SDS-PAGE |
| Endotoxin Level | < 1 EU/μg of protein by gel clotting method |
| Biological Activity | ED50 < 0.6 ng/ml, measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts in serum-free medium and CellTiter-Glo cell viability assay, corresponding to a specific activity of > 1.7 × 106 U/mg. |
| Expression System | E. coli |
| Apparent Molecular Weight | ~6.2 kDa, on SDS-PAGE under reducing conditions. |
| Formulation | Lyophilized after extensive dialysis against 10 mM phosphate buffer, pH 7.0, 200 mM NaCl buffer. |
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
| Storage & Stability | Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |