| Species | Mouse |
| Protein Construction | Expressed with an N-terminal Met. | TNF-α (Leu80-Leu235)Accession # P06804 |
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| Purity | > 98% as analyzed by SDS-PAGE> 98% as analyzed by HPLC |
| Endotoxin Level | < 1 EU/μg of protein by LAL method |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg in the presence of actinomycin D. |
| Expression System | E. coli |
| Theoretical Molecular Weight | 17.4 kDa |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.2. |
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. |
| Storage & Stability | Upon receiving, this product remains stable for 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. Avoid repeated freeze-thaw cycles. |