Z02691 EGF, Human

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Z02691 EGF, Human

产品简介
SpeciesHuman
Protein Construction

Expressed with an N-terminal Met.

EGF (Asn971-Arg1023)Accession # P01133
Purity> 95% as analyzed by SDS-PAGE
Endotoxin Level< 1 EU/μg of protein by gel clotting method
Biological ActivityED50 < 0.6 ng/ml, measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts in serum-free medium and CellTiter-Glo cell viability assay, corresponding to a specific activity of > 1.7 × 106 U/mg.
Expression SystemE. coli
Apparent Molecular Weight~6.2 kDa, on SDS-PAGE under reducing conditions.
FormulationLyophilized after extensive dialysis against 10 mM phosphate buffer, pH 7.0, 200 mM NaCl buffer.
ReconstitutionIt is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage & StabilityUpon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.


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