Z02774 TNF-α, Mouse

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Z02774 TNF-α, Mouse

产品简介
SpeciesMouse
Protein Construction

Expressed with an N-terminal Met.

TNF-α (Leu80-Leu235)Accession # P06804
Purity> 98% as analyzed by SDS-PAGE> 98% as analyzed by HPLC
Endotoxin Level< 1 EU/μg of protein by LAL method
Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg in the presence of actinomycin D.
Expression SystemE. coli
Theoretical Molecular Weight17.4 kDa
FormulationLyophilized from a 0.2 μm filtered solution in PBS, pH 7.2.
ReconstitutionIt is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.
Storage & StabilityUpon receiving, this product remains stable for 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. Avoid repeated freeze-thaw cycles.


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