A linker is an amino acid chain that connects two fused proteins, allowing for the appropriate separation of protein functional domains or the maintenance of necessary inter-domain interactions. The most widely known flexible linker is the poly-Glycine-Serine (GS) linker. The GS Linker includes various forms, such as (GS)n, (GGS)n, (GGGS)n, (GGGGS)n, etc. These GS linkers are widely used in connecting bispecific antibodies, antibody fusion proteins, single-chain antibodies, peptides and ADCs, etc.
GenScript’s Anti-GS Linker Affinity Resin FF (Cat. No. L01036) is designed for purification of proteins, antibodies and compounds containing (GnS)m linker (n≥2, m≥2) from commonly used protein expression systems such as bacteria, yeast and mammalian cells. The GS linker fusion protein in cell lysate can specifically bind to the anti-GS linker antibody coupled to the resin. Nonspecific binding reactions are eliminated by stringent washing steps, and the purified target protein is eluted with a high recovery rate. Table 1 lists the main characteristics of Anti-GS Linker Affinity Resin FF.
Table 1. Characteristics of Anti-GS Linker Affinity Resin FF| Product content | 50% settled resin in TBS with 0.1% Procin300 | | Matrix | 4% highly cross-linked agarose | | Average bead size | 90 μm | | Ligand | Anti-GS Linker Antibody | | Binding capacity | ≥4 mg antibodies containing (G4S)3 linker per mL settled resin
| | Specificity | Proteins, antibodies and compounds containing (GnS)m linker (n≥2, m≥2), such as (G4S)3 linker, (G2S)2 linker, (G2S)4 linker, (G3S)3 linker and (G4S)2 linker, etc.
| | Storage and stability | Store at 2-8 °C for up to 12 months. Do not freeze the resin. | | Resin reuse | When properly regenerated and stored according to the instructions, the resin can be reused up to 10 times without significant loss of binding capability. | | Elution method | Acidic elution (pH 3.0) | | Reagent compatibility | Compatible with commonly used lysis reagents. For reagents compatible with purifications, see tables 2 and 3. |
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