L00436 His Tag ELISA Detection Kit

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L00436 His Tag ELISA Detection Kit

产品详细

Note

L00436 interferes with IgG in mouse/rat serum and is not suitable for detecting mouse/rat serum samples.

产品简介

Product Description

GenScript His Tag ELISA Detection Kit, a 1.5 hour competition ELISA, is developed for rapid and high throughput detection of His-tagged proteins. There are several potential usages:
●Quickly identify the presence of His-tagged proteins in samples.
●Optimize protein expression by monitoring the His-tagged proteins level.
●High throughput screening of stable cell lines expressing His-tagged proteins.

Detection Range

1 ng/ml~729 ng/ml

Sensitivity

1 ng/ml His-tagged proteins

Test Samples

N-terminal/C-terminal/internal His-tagged proteins4 x His/5 x His/6 x His-tagged proteins
Mammalian, yeast and bacteria cell lysates or cell supernatant

Conveniency

Provide all reagents required for test
Complete test within 1.5 hours

Reagents compatibility

Tolerable with various reagents at certain concentration

Storage

The unopened kit is stable for 12 months if stored at 2-8 °C and the opened kit may be stable for 1 month at 2-8 °C. Do not freeze the kit.

Kit Contents

Component	Quantity	Part. No
His Tag Plate	1 plate (8 wells × 12 strips)	436-80
Anti-His Monoclonal Antibody	6 ml	436-20
Antibody Tracer	12 ml	436-30
His Tag Standards (0,1,3,9,27,81,243,729 ng/ml)	1 ml per each standard	436-11,12,13,14,15,16,17,18
Assay Diluent	60 ml	436-60
20 × Wash Solution	40 ml	B-A1-70
TMB Solution	12 ml	B-A1-40
Stop Solution	6 ml	B-A1-50
His Tag Standard Stock (10 μg/ml)	500 μl	436-10
Plate Sealer	2	N/A

图例

Concentration gradient represents X, OD450 value represents Y, all X values will be transformed to Log(X) to create the standard curve which was calculated by 4PL (Figure 1). The operator can get log values of sample concentration by inputting the OD450 value of unknown samples. By formula X=10X, sample concentration can be calculated.»

The intra-assay precision of the L00436 is less than 10%, and the inter-assay precision is less than 15%.»

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

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